Those variables are  easy to tune.

The evidence popup here between 128 and 256K for in memory workload.

My SQL SQL layer is optimistic and think that data will come from the storage engine at the speed of the memory bus , but in practice for IO bound workload and range scan in the table order it could not be the case , for MyISAM when reading from tables on disk, IOs are aligned on the read buffer so it may be of an intrest to increase it here.

In myisam.cc my_default_record_cache_size is set to  global_system_variables.read_buff_size;

To show IO size :
strace -p id_mysqld -e read -e write

If  monitoring smaller IO  request size from iostat  -xm, it may be that you are …