One of my favorite tools in the Percona Toolkit is pt-query-digest. This tool is indispensable for identifying your top SQL queries, and analyzing which queries are accounting for your database load.
But the report you get from pt-query-digest is only as good as the log of queries you give it as input. You need a large enough sample of query logs, collected over a period of time when you have representative traffic on your database.
You also need the log to include all the queries, not just those that take more than N seconds. The reason is that some queries are individually quick, and would not be logged if you set the long_query_time
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